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KMID : 1007519990080030189
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Food Science and Biotechnology
1999 Volume.8 No. 3 p.189 ~ p.192
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The Separation of Major Flavonoglycosides from Ginkgo biloba L . Leaves by High - Speed Counter - current Chromatography
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Lee, Chang ho
LEE, CHERL HO/Lee, Boo Yong
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Abstract
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Flavonoglycosides were fractionated from ginkgo (Ginkgo biloba L.) leaves by high-speed countercurrent chromatography (HSCCC). A series of preliminary studies was performed to optimize the physical properties of the two-phase solvent system and the operating parameters including the rotation speed of the column. elution mode of the mobile phase, and flow rate using a commercial multilayer coil HSCCC centrifuge. The best experimental conditions were found to be as follows: solvent system: chloroform / methanol / water (40:39:21, v/v/v); rotation speed: 800 rpm; flow rate: 3 mL/min; elution mode: upper phase in the tail to head mode or lower phase in the head to tail mode; sample size: 100 μL. ISCCC fractions were hydrolyzed and three major aglycones from the corresponding glycosides, i.e., quercetin, kaempferol, and isorhamnetin, were checked by HPLC analysis. The recovery ratio of these flavonoglycosides from dried ginkgo leaves extract were 83.3% with the upper mobile phase and 24.4% with the lower mobile phase.
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